MUSKMELON (Cucumis melo L.) HYBRIDS

 

I. Application and Amplification of General Seed Certification Standards

A. The General Seed Certification Standards are basic and together with the following specific standards 

     constitute the standards for certification of hybrid muskmelon seed.

B. The General standards are amplified as follows to apply specifically to the hybrids of muskmelon:

1. Eligibility requirements for certification

(a) An inbred / The parental line to be eligible for certification shall be from a source such that its identity may 

     be assured and approved by the Certification Agency.

(b) Hybrid seed to be eligible for certification shall be the progeny of two approved  inbred / parental lines one 

     of which shall be male sterile.

2. Classes and sources of seed

(a) An inbred /parental line shall be a relatively true breeding strain resulting from self -pollination with selection.

*(b) The  Foundation class seed shall consist of an approved male sterile line (genetic- male sterility) to be used as a female parent and an approved parental line to be used as a male parent for the purpose of producing hybrid seed.

*(c) A male sterile line shall be a monogenic recessive strain carrying genetic-male sterility, which sheds no viable pollen and is maintained by the monogenic dominant heterozygous strain and is used as maintainer line in propagation blocks.

(d) The Certified class seed shall be the hybrid seed to be planted for any use except seed production .

(*Applicable if male sterile line is used).

 

II. Land requirements

Land to be used for seed production of hybrid muskmelon shall be free of volunteer plants.   

 

III. Field Inspection

A. Foundation seed

A minimum of four inspections shall be made as follows:

1. the first inspection shall be made before flowering in order to determine isolation, volunteer plants and other 

    relevant factors;

2. the second and third inspections shall be made during flowering and fruiting stage to cheek isolation, 

    offtypes, and also fertile segregants in seed parent if a male sterile line is used;

3. the fourth inspection shall be made at mature fruit stage and prior to harvesting in order to verify the true 

    characteristics of the fruits and other relevant factors.

 

B. Certified seed

A minimum of four inspections shall be made as follows: 

1. the first inspection shall be made before flowering in order to determine isolation, volunteer plants, out crosses  demarcation between seed parent and pollinator blocks or  planting ratio, as the case may be, and errors in planting.

2. the second and third inspections shall be made during flowering and fruiting stage to cheek isolation, offtypes, accuracy in emasculation (in case andromonoecious line is used) or fertile segregants (pollen shedding cymes which are likely to appear in 1:1 ratio) in case sterile line is used and other relevant factors;

3. the fourth inspection shall be made at mature fruit stage and prior to harvesting in order to verify the true characteristics of the fruits and other relevant factors.

    

IV. Field Standards

A. General requirements

1. Isolation

Seed fields shall be isolated from the contaminants shown in the column 1 of the Table below by the distances specified in columns 2 and 3 of the said Table:


        Contaminants                                                                                   Minimum distance (meters)


                                                                                                                Foundation        Certified


            1                                                                                                         2                     3


Fields of other varieties including commercial 

hybrid of the same variety                                                                             1500                1000

Fields of the same hybrid (code designation) not conforming to 

varietal purity requirements for certification and from

 longmelon Cucumis melo L.var utilissimus Duth. & Full.), 

oriental pickling melon (Cucumis melo L. var. conomon Makino),

snapmelon phnoot: Cucumis melo L.var. momordica

Duth.& Full., Cucumis prophetarum; weedmelon (Takmek) 

(Cucumis melo (L.) Var. agrestis Naud. and other non-dessert

forms of Cucumis melo (L.) known to cross or suspected 

of being able to cross                                                                                   1500                1000  

Between blocks of the parental lines incase seed parent

and pollinator are planted in separate blocks and hand 

pollination is to be adopted                                                                               -                      5


 

B. Specific requirements


        Factor                                                                                              Maximum permitted (%)*


                                                                                                                Foundation            Certified


        Offtypes in seed parent                                                                           0.010                  0.050

        Offtypes in pollinator                                                                                -                        0.050

        **Fertile segregants (Pollen shedding cymes) in seed parent                    0.050                  0.10      

        Male flowers shedding pollens in seed parent  if 

        andromonoecious line is used                                                                    -                       0.10    

        @ Objectionable weed plants                                                                 None                 None

        @@ Plants affected by seed borne disease                                              0.10                   0.20  


*    Standards for offtypes and objectionable weeds shall be met at any inspection conducted at and after flowering and for seed borne disease at final inspection.

** Applicable if male sterile line is used.

@ Objectionable weed shall be : Cucumis prophetarum; Weedmelon (Takmek): (Cucumis melo (L.) Var. agrestis Naud. and other non-dessert forms of Cucumis melo (L.)

@@ Seed born disease shall be : Cucumber Mosaic Virus (CMV).    

 

V. Seed standards


        Factor                                                                                              Standards for each class


                                                                                                                Foundation            Certified


Pure seed (minimum)                                                                                98.0%                    98.0%

Inert matter (maximum)                                                                              2.0%                       2.0%

Other crop seeds (maximum)                                                                     5/ kg                     10 / kg

Total Weed seeds (maximum)                                                                   None                      None

* Objectionable weed seed (maximum)                                                     None                      None

Germination (minimum)                                                                              60%                        60%

Moisture (maximum)                                                                                  7.0%                       7.0%

For vapour-proof containers (maximum)                                                    6.0%                       6.0%


* Objectionable weeds are the same as given at IV. B. above.

(a) All Certified class seed lots which have been produced by adopting emasculation shall be subjected to grow-out test and shall conform to the following minimum genetic purity requirements:

 


                            Class                                                                Genetic purity (%) (minimum)


                        Certified                                                                                90.0*


    *During grow-out test, the offtype plants (other than selfed plants) such as segregants, outcrosses and plants

     of other varieties should not exceed more than 1.50%out of the 10.0% plants earmarked for selfed plants.

 

(b) The minimum population size of 400 plants shall be maintained in two replicates of 200 each or four of 100

     each through out the test and each plant shall be examined individually. The reject number shall be as follow:


                  Class                                                           Genetic purity (%)                Reject number

                                                                                        (minimum)


               Certified                                                            90.0 (10 in 100)                        44